Pseudocolor any Grayscale Image

This function pseudocolors any grayscale image to custom colormap. An optional mask can leave background out in the pseudocolored image. Additionally, optional maximum and minimum values can be specified. When pcv.params.debug='print' then the image gets saved to pcv.params.debug_outdir, andpcv.params.dpi can be set for the image that gets saved. If unaltered, the matplotlib default is 100 pixels per inch.

plantcv.visualize.pseudocolor(gray_img, obj=None, mask=None, background="image", cmap=None, min_value=0, max_value=255, axes=True, colorbar=True, obj_padding='auto')

returns pseudocolored image (that can be saved with pcv.print_image)

  • Parameters:

    • gray_img - Grayscale image data
    • obj - ROI or plant contour object (optional) if provided, the pseudocolored image gets cropped down to the region of interest.
    • mask - Binary mask made from selected contours
    • background - Background color/type. Options are "image" (gray_img), "white", or "black". A mask must be supplied.
    • cmap - Custom colormap, see here for tips on how to choose a colormap in Matplotlib.
    • min_value - Minimum value (optional) for range of the colorbar.
    • max_value - Maximum value (optional) for range of the colorbar.
    • axes - If False then the title, x-axis, and y-axis won't be displayed (default axes=True).
    • colorbar - If False then the colorbar won't be displayed (default colorbar=True)
    • obj_padding - if "auto" (default) and an obj is supplied, then the image is cropped to an extent 20% larger in each dimension than the object. A single integer is also accepted to define the padding in pixels.
  • Context:

    • Used to pseudocolor any grayscale image to custom colormap
  • Example use:

Original grayscale image

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Mask

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from plantcv import plantcv as pcv

pcv.params.debug='plot'

# Pseudocolor an image with 'viridis' colormap
pseudo_img = pcv.visualize.pseudocolor(gray_img=img, obj=None, mask=None, cmap='viridis',
                                       min_value=0, max_value=255)

# Pseudocolor the same image but include the mask and limit the range of values
pseudo_img_masked = pcv.visualize.pseudocolor(gray_img=img, obj=None, mask=mask,
                                              background="white", cmap='viridis',
                                              min_value=30, max_value=200)

# Save the masked and pseudocolored image
pcv.print_image(pseudo_img_masked, 'nir_tv_z300_L1_pseudocolored.png')

# Pseudocolor the masked area and plot on the grayscale background
pseudo_img_on_input = pcv.visualize.pseudocolor(gray_img=img, obj=None, mask=mask,
                                                background="image", cmap="viridis")

# Print out a pseudocolored image with cropping enabled, axes disabled.
pcv.params.debug='print'
pseudo_crop_no_axes = pcv.visualize.pseudocolor(gray_img=img, obj=obj, mask=mask,
                                                background="white", cmap='viridis',
                                                axes=False, obj_padding = 'auto')

# Use a black background instead
pseudo_img_black_bkgd = pcv.visualize.pseudocolor(gray_img=img, obj=None, mask=mask,
                                                  background="black", cmap='viridis')

simple_pseudo_img = pcv.visualize.pseudocolor(gray_img=img, obj=None, mask=mask,
                                              background="image", axes=False,
                                              colorbar=False, cmap='viridis')

Pseudocolored Image

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Pseudocolored, background="white"

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Pseudocolored, background="image"

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Pseudocolored, Cropped, Disabled Axes Image

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Pseudocolored, background="black"

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Pseudocolored, Plotted on Input Image (no axes or colorbar)

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