Image Subtract¶

This is a function is used to subtract values of one gray-scale image array from another gray-scale image array. The resulting gray-scale image array has a minimum element value of zero. That is all negative values resulting from the subtraction are forced to zero.

plantcv.image_subtract(gray_img1, gray_img2)

returns new_img

• Parameters:
  • gray_img1 - Grayscale image data from which gray_img2 will be subtracted
  • gray_img2 - Grayscale image data to be subtracted from gray_img1
• Context:
  • returns difference in pixel values of two images
• Example use:
  • Below

Gray_Img1

Gray_Img2

from plantcv import plantcv as pcv

# Set global debug behavior to None (default), "print" (to file), 
# or "plot" (Jupyter Notebooks or X11)
pcv.params.debug = "plot"

# Subtract image from another image. 
subtracted_img = pcv.image_subtract(gray_img1, gray_img2)

Result

Source Code: Here