Analyze Color

Extract color data of objects and produce a histogram. Data can be extracted for RGB (Red, Green, Blue), HSV (Hue, Saturation, Value) and LAB (Lightness, Green-Magenta, Blue Yellow) channels.

plantcv.analyze.color(rgb_img, labeled_mask, n_labels=1, colorspaces="hsv", label=None)

returns Ridgeline plot of histograms of hue values

  • Parameters:
    • rgb_img - RGB image data
    • labeled_mask - Labeled mask of objects (32-bit, output from pcv.create_labels or pcv.roi.filter).
    • n_labels - Total number expected individual objects (default = 1).
    • colorspaces - 'all', 'rgb', 'lab', or 'hsv'. This can limit the data saved out (default = 'hsv').
    • label - Optional label parameter, modifies the variable name of observations recorded. Can be a prefix or list (default = pcv.params.sample_label).
  • Context:
    • Used to extract color data from RGB, LAB, and HSV color channels.
    • Generates histogram of color channel data.
  • Example use:
  • Output data stored: Data ('blue_frequencies', 'green_frequencies', 'red_frequencies', 'lightness_frequencies', 'green-magenta_frequencies', 'blue-yellow_frequencies', 'hue_frequencies', 'saturation_frequencies', 'value_frequencies', 'hue_circular_mean', 'hue_circular_std', 'hue_median') automatically gets stored to the Outputs class when this function is ran. These data can always get accessed during a workflow (example below). For more detail about data output see Summary of Output Observations

Original image

Screenshot


from plantcv import plantcv as pcv

# Set global debug behavior to None (default), "print" (to file), 
# or "plot" (Jupyter Notebooks or X11)

pcv.params.debug = "plot"
# Optionally, set a sample label name
pcv.params.sample_label = "plant"

# Analyze Color
analysis_image = pcv.analyze.color(rgb_img=rgb_img, labeled_mask=mask, n_labels=1, colorspaces='hsv')

# Access data stored out from analyze_color
hue_circular_mean = pcv.outputs.observations['plant_1']['hue_circular_mean']['value']

Histograms of hue values

Screenshot

Source Code: Here